2023 Early Hearing Detection & Intervention Conference
March 5-7, 2023 • Cincinnati, OH
5/24/2021 | 8:30 AM - 10:30 AM | Neuromuscular anatomy of Chironomus sp. '' Florida '': potential model to study toxicity in aquatic environments | Virtual Platform
Neuromuscular anatomy of Chironomus sp. '' Florida '': potential model to study toxicity in aquatic environments
Different markers have been used to detect toxicity in freshwater environments using chironomid larvae. Cellular markers could help to detect toxicity during early stages, anticipating effects at morphological or behavioral levels. The objective of this study is to propose the neuromuscular tissue of Chironomus sp. Florida as a cellular marker. Since this tissue is not known in chironomids, we have described its anatomy by using immunohistochemistry and confocal microscopy to produce anatomical diagrams. Analyzing the first abdominal segment, a total of 62 muscles were described. The ganglion corresponding to this segment has two tracts whose nerves branch into axons that innervate each muscle through a terminal composed of numerous synaptic boutons. The response of one of these terminals was evaluated against temperature, sex and age. Larvae reared at 30ºC were observed to have a higher density of synaptic boutons when compared to larvae reared at 25ºC, something not observed at different ages or sex. Although this study is in a development stage to determine if the studied structures could be used as toxicity markers, it is clear that they are responsive, and their changes are easy to quantify.
- Biological effects
- Interdisciplinary
- Nature-based solutions
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Presenters/Authors
Roberto Reyes-Maldonado
(), University of Puerto Rico, Rio Piedras, robertoomaldo@gmail.com;
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Alonso Ramírez
(), North Carolina State University, alonso.ramirez@ncsu.edu;
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Bruno Marie
(), University of Puerto Rico, Institute of Neurobiology, brunomariemail@gmail.com;
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