2023 Early Hearing Detection & Intervention Conference
March 5-7, 2023 • Cincinnati, OH
5/20/2019 | 11:30 AM - 11:45 AM | BIOLOGICAL INTERFERENCE IN WASTEWATER TOXICITY TESTS AND POTENTIAL INFLUENCE OF HIGHER AMBIENT TEMPERATURES AND EXTENDED WARM PERIODS | 251 AB
BIOLOGICAL INTERFERENCE IN WASTEWATER TOXICITY TESTS AND POTENTIAL INFLUENCE OF HIGHER AMBIENT TEMPERATURES AND EXTENDED WARM PERIODS
Facilities discharging treated wastewater must meet certain water quality limits as defined in their permit(s). Often the parameters include whole effluent toxicity (WET) tests, which may be acute or short-term chronic (STC). Cases of significant WET failure with both Ceriodaphnia dubia and Pimephales promelas in effluents have been traced to the presence of microorganisms in the effluent. To track down the suspect toxicant, Toxicity Identification Evaluation (TIE) methods were modified by inclusion of manipulations effective in reducing or eliminating biological interference (e.g., UV, chlorination). Wastewater samples were cultured in an aerobic environment using, primarily, MacConkey, TSA, and Columbia agars. While several bacterial taxa have been identified in wastewater, Pseudomonas and Aeromonas are the prime suspects in causing significant biological interference. Significant effects to the test organisms were replicated by reintroduction of microorganisms into clean lab water. Biological interference in WET tests generally disappears once air temperatures drop in the autumn. Recent cases, however, have occurred later in the year when it is suspected that higher, sustained temperatures have allowed microorganisms to continue to flourish either in the discharged wastewater itself and/or on surfaces in the wastewater plant.
- Water Quality
- Microbial
- Temperature
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Presenters/Authors
David Pillard
(), TRE Environmental Strategies, pillardda.tre@gmail.com;
ASHA DISCLOSURE:
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Nonfinancial -
Rami Naddy
(), TRE Environmental Strategies, naddyrb.tre@gmail.com;
ASHA DISCLOSURE:
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Nonfinancial -
Rebecca Gagnon
(), TRE Environmental Strategies, jenschra.tre@gmail.com;
ASHA DISCLOSURE:
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Nonfinancial -