2023 Early Hearing Detection & Intervention Conference

March 5-7, 2023 • Cincinnati, OH

5/24/2021 | 2:00 PM - 3:30 PM | On-site/ultra-rapid eDNA detection method for stream invasive fish | Virtual Platform

On-site/ultra-rapid eDNA detection method for stream invasive fish

Environmental DNA method has been developed for biomonitoring in aquatic habitats. Recently, the eDNA methods were applied to fish distribution analysis in freshwater ecosystems. However, the eDNA collection/measurement will take a time, e..g., 5-6 hours. We recently developed a on-site/rapid method for the field processing of eDNA samples and measurements using an ultra-rapid mobile PCR (hereafter, mobile PCR) to reduce the measurement time to 30 min and maintain high detectability of aquatic organisms. We firstly tested the ability and performance of the on-site/rapid method with comparing to the laboratory experiment using standard DNA. Further, we demonstrated its on-site use to detect the distribution of silver carp, Hypophthalmichthys molitrix, an invasive fish in Japanese rivers and lakes. We compared the on-site eDNA measurement to the laboratory extraction and detection using a benchtop qPCR platform and the national survey to confirm the performance. Our on-site method reduced the measurement time to 30 min and provided high detectability of aquatic organisms compared to the national observation surveys and laboratory eDNA method. We summarized that our on-site/rapid eDNA method could be immediately applied to various taxa and environments in freshwater habitats.

Conservation
Genomics
Stream

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Presenters/Authors

Hideyuki Doi (), University of Hyogo, hideyuki.doi@icloud.com;