2023 Early Hearing Detection & Intervention Conference
March 5-7, 2023 • Cincinnati, OH
5/22/2018 | 9:15 AM - 9:30 AM | USE OF METAGENOMICS DATA FOR MONITORING: PROMISES AND CHALLENGES | 330 A
USE OF METAGENOMICS DATA FOR MONITORING: PROMISES AND CHALLENGES
Metagenomics data have the potential for revolutionizing biological assessment. The possible advantages of this data over conventional morphological identifications include a more complete census of organisms at a site and more consistent identifications of obscure taxa, both of which may substantially reduce sampling variability. We report here on analysis of periphyton samples collected from ~400 sites across the United States. At each site, periphyton were sequenced and a conventional diatom sample was identified. Analysis of these data suggest that use of metagenomic data stabilized, but did not reduce variability in community composition over multiple visits. Detailed comparisons between morphological and metagenomic data with regard to responses to environmental gradients were further hindered by a large number of sequences that did not match up with reference sequences in existing databases, which, in turn, resulted in a large number of distinct taxa in the genomic data that could be assigned to broad taxonomic groups.
- Metagenomics
- Periphyton
- Assessment
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Presenters/Authors
Lester Yuan
(), U.S. Environmental Protection Agency, yuan.lester@epa.gov;
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Richard Mitchell
(), U.S. Environmental Protection Agency, mitchell.richard@epa.gov;
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Erik Pilgrim
(), U.S Environmental Protection Agency, pilgrim.erik@epa.gov;
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