2023 Early Hearing Detection & Intervention Conference

March 5-7, 2023 • Cincinnati, OH

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5/20/2019 | 2:15 PM - 2:30 PM | TRACING THE SOURCES, FATES, AND ACTIVITIES OF LEAF-ASSOCIATED MICROBES INTO THE STREAM FOOD WEB | 151 ABC

TRACING THE SOURCES, FATES, AND ACTIVITIES OF LEAF-ASSOCIATED MICROBES INTO THE STREAM FOOD WEB

Quantitative stable isotope probing (qSIP) combined with high throughput sequencing provides a new tool for differentiating active versus inactive microbial taxa throughout leaf litter decomposition. Prior research has demonstrated this technique using 16S rRNA gene sequencing to differentiate active versus inactive bacterial and archeal taxa. We demonstratethat qSIP combined with sequencing the internal transcribed spacer region (ITS) of rRNA genes can be used to identify fungal taxa that are actively growing on decomposing leaves. We applied this approach to a litter decomposition experiment in which four leaf species were incubated in stream water for up to 24 days. Results show that most active fungi colonize with the leaf and that many fungal taxa are present but not active. Furthermore, bacteria colonize from the water column, and most are active. We also used qSIP to trace the fates of bacterial taxa ingested with leaf litter by a shredding caddisfly. We found evidence for 'gut specialists' that are active only when fed certain leaf types. Cottonwood gut specialists outnumber oak gut specialists.

Microbial
Organic Matter
Bacteria

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Presenters/Authors

Benjamin Koch (), Northern Arizona University, ben.koch@nau.edu;