2023 Early Hearing Detection & Intervention Conference

March 5-7, 2023 • Cincinnati, OH

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6/05/2017  |   2:15 PM - 2:30 PM   |  EXAMINATION OF THE EFFECTS OF METALS ON BENTHIC ALGAE IN STREAMS OF DIFFERENT TROPHY USING A FIELD BIOASSAY   |  306B

EXAMINATION OF THE EFFECTS OF METALS ON BENTHIC ALGAE IN STREAMS OF DIFFERENT TROPHY USING A FIELD BIOASSAY

It is difficult to isolate effects of metals and acids in situ in streams impacted by mining. We examined the efficacy of using chemical diffusing substrates (CDS) to determine how acid, Fe3+, Mn2+, Al3+ and nutrient regime interactions affect benthic algae. CDS containing Mn (24 mg/L), Fe (24 mg/L), Al (6.4 mg/L), acid (1.0N H2SO4), Mn+acid, Fe+acid, and Al+acid were deployed in a flow-through system to determine flux from the liquid media. Flux values ranged from 0.5 to 7.4 ug/cm2/day. Five replicates of each CDS type, and a water control, were placed into a eutrophic (ES) and an oligotrophic (OS) stream. In OS, algal cell densities were significantly lower in treatments with acid. Densities were about 5 times higher in ES, but did not differ significantly among treatments. Treatments with acid had significantly lower chlorophyll and higher phaeophytin concentrations in ES. Acid-tolerant taxa were significantly more abundant for treatments with acid in OS but not ES. Algae were more affected by acid than metals. Higher alkalinity and nutrients in ES may have buffered acid effects, reducing impacts on algae.

  • C13 Ecotoxicology
  • S03 Stressing the 'Eco' in Freshwater Ecotoxicology
  • C01 Algae & C24 Eutrophication

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Presenters/Authors

Dean DeNicola (), Slippery Rock University, dean.denicola@sru.edu;


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Aaron Onufrak (), Slippery Rock University, onufrak.2@wright.edu;


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Michael Stapleton (), Slippery Rock University, michael.stapleton@sru.edu;


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